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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
21/03/2022 |
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Data da última atualização: |
22/03/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GALLENTI, R.; HUSSEIN, H. E.; ALZAN, H. F.; SUAREZ, C. E.; UETI, M.; ASURMENDI, S.; BENITEZ, D.; ARAUJO, F. R.; ROLLS, P.; SIBEKO-MATJILA, K.; SCHNITTGER, L.; FLORIN-CHRISTENSEN, M. |
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Afiliação: |
ROMINA GALLENTI, Instituto Nacional de Tecnología Agropecuaria-Consejo Nacional de Investigaciones Científicas y Técnicas (INTA-CONICET), Argentina.; HALA E. HUSSEIN, Washington State University, USA.; HEBA F. ALZAN, Washington State University, USA.; CARLOS E. SUAREZ, Washington State University, USA.; MASSARO UETI, Washington State University, USA.; SEBASTIÁN ASURMENDI, Instituto Nacional de Tecnología Agropecuaria-Consejo Nacional de Investigaciones Científicas y Técnicas (INTA-CONICET), Argentina.; DANIEL BENITEZ, Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina.; FLABIO RIBEIRO DE ARAUJO, CNPGC; PETER ROLLS, Department of Agriculture & Fisheries, Tick Fever Centre, Australia.; KGOMOTSO SIBEKO-MATJILA, University of Pretoria, South Africa.; LEONHARD SCHNITTGER, Instituto Nacional de Tecnología Agropecuaria-Consejo Nacional de Investigaciones Científicas y Técnicas (INTA-CONICET), Argentina.; MÓNICA FLORIN-CHRISTENSEN, Instituto Nacional de Tecnología Agropecuaria-Consejo Nacional de Investigaciones Científicas y Técnicas (INTA-CONICET), Argentina. |
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Título: |
Unraveling the complexity of the Rhomboid Serine Protease 4 Family of Babesia bovis using bioinformatics and experimental studies. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Pathogens, v. 11, n. 344, 2022. |
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Idioma: |
Inglês |
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Conteúdo: |
Babesia bovis, a tick-transmitted apicomplexan protozoon, infects cattle in tropical and subtropical regions around the world. In the apicomplexans Toxoplasma gondii and Plasmodium falciparum, rhomboid serine protease 4 (ROM4) fulfills an essential role in host cell invasion. We thus investigated B. bovis ROM4 coding genes; their genomic organization; their expression in in vitro cultured asexual (AS) and sexual stages (SS); and strain polymorphisms. B. bovis contains five rom4 paralogous genes in chromosome 2, which we have named rom4.1, 4.2, 4.3, 4.4 and 4.5. There are moderate degrees of sequence identity between them, except for rom4.3 and 4.4, which are almost identical. RT-qPCR analysis showed that rom4.1 and rom4.3/4.4, respectively, display 18-fold and 218-fold significantly higher (p < 0.01) levels of transcription in SS than in AS, suggesting a role in gametogenesis-related processes. In contrast, transcription of rom4.4 and 4.5 differed non-significantly between the stages. ROM4 polymorphisms among geographic isolates were essentially restricted to the number of tandem repeats of a 29-amino acid sequence in ROM4.5. This sequence repeat is highly conserved and predicted as antigenic. B. bovis ROMs likely participate in relevant host?pathogen interactions and are possibly useful targets for the development of new control strategies against this pathogen. |
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Palavras-Chave: |
Inter-strain polymorphism; Rhomboid serine proteases. |
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Thesagro: |
Babesia Bovis. |
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Thesaurus Nal: |
Babesiosis; Gene expression; Tick-borne diseases. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/232800/1/2022-Babesia-ROM4.pdf
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Marc: |
LEADER 02337naa a2200325 a 4500
001 2141112
005 2022-03-22
008 2022 bl uuuu u00u1 u #d
100 1 $aGALLENTI, R.
245 $aUnraveling the complexity of the Rhomboid Serine Protease 4 Family of Babesia bovis using bioinformatics and experimental studies.$h[electronic resource]
260 $c2022
520 $aBabesia bovis, a tick-transmitted apicomplexan protozoon, infects cattle in tropical and subtropical regions around the world. In the apicomplexans Toxoplasma gondii and Plasmodium falciparum, rhomboid serine protease 4 (ROM4) fulfills an essential role in host cell invasion. We thus investigated B. bovis ROM4 coding genes; their genomic organization; their expression in in vitro cultured asexual (AS) and sexual stages (SS); and strain polymorphisms. B. bovis contains five rom4 paralogous genes in chromosome 2, which we have named rom4.1, 4.2, 4.3, 4.4 and 4.5. There are moderate degrees of sequence identity between them, except for rom4.3 and 4.4, which are almost identical. RT-qPCR analysis showed that rom4.1 and rom4.3/4.4, respectively, display 18-fold and 218-fold significantly higher (p < 0.01) levels of transcription in SS than in AS, suggesting a role in gametogenesis-related processes. In contrast, transcription of rom4.4 and 4.5 differed non-significantly between the stages. ROM4 polymorphisms among geographic isolates were essentially restricted to the number of tandem repeats of a 29-amino acid sequence in ROM4.5. This sequence repeat is highly conserved and predicted as antigenic. B. bovis ROMs likely participate in relevant host?pathogen interactions and are possibly useful targets for the development of new control strategies against this pathogen.
650 $aBabesiosis
650 $aGene expression
650 $aTick-borne diseases
650 $aBabesia Bovis
653 $aInter-strain polymorphism
653 $aRhomboid serine proteases
700 1 $aHUSSEIN, H. E.
700 1 $aALZAN, H. F.
700 1 $aSUAREZ, C. E.
700 1 $aUETI, M.
700 1 $aASURMENDI, S.
700 1 $aBENITEZ, D.
700 1 $aARAUJO, F. R.
700 1 $aROLLS, P.
700 1 $aSIBEKO-MATJILA, K.
700 1 $aSCHNITTGER, L.
700 1 $aFLORIN-CHRISTENSEN, M.
773 $tPathogens$gv. 11, n. 344, 2022.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
28/04/2023 |
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Data da última atualização: |
28/04/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 3 |
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Autoria: |
SPASIANI, P. P.; HOMEM, B. G. C.; LIMA, I. B. G. de; GUIMARAES, B. C.; MEDEIROS, E. S. de; MUIR, J. P.; OLIVEIRA, M. S. de; BODDEY, R. M.; CASAGRANDEM, D. R. |
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Afiliação: |
PAOLA PALAURO SPASIANI, UFLA; BRUNO GROSSI COSTA HOMEM, UFLA; ITALO BRAZ GONCALVES DE LIMA, UFLA; BIANCA COSTA GUIMARAES, UFLA; ELIAS SILVA DE MEDEIROS, UNIVERSIDADE FEDERAL DA GRANDE DOURADOS; JAMES PIERRE MUIR, TEXAS A&M UNIVERSITY; MARCELO SILVA DE OLIVEIRA, UFLA; ROBERT MICHAEL BODDEY, CNPAB; DANIEL RUME CASAGRANDE, UFLA. |
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Título: |
Light competition is the key factor determining spatio-temporal variability in legume proportion within Marandu palisadegrass-forage peanut mixed pastures. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Crop & Pasture Science, Published online: 21 March 2023. |
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ISSN: |
1836-0947 |
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DOI: |
10.1071/CP22134 |
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Idioma: |
Inglês |
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Conteúdo: |
Context Understanding ecology in grass-legume pastures can help support strategies aimed at maintaining canopy stability in terms of botanical composition.Aims This 2-year study evaluated spatial variability, focusing on plant structural characteristics in a Marandu palisadegrass [Urochloa brizantha (Hochst. Ex A. Rich.) R.D. Webster cv. Marandu]-forage peanut (Arachis pintoi Krapov. and W.C. Greg. cv. BRS Mandobi) mixed canopy under grazing in continuous stocking management.Methods The pasture was managed with canopy height of 20-25 cm. The experimental area had a permanent sample grid containing 50 pre-established and georeferenced plots. Principal component analysis was performed, and spatial dependence structure of the first principal component and structural variables were separately characterised by geostatistical analysis.Key results There was spatial dependence of the structural characteristics, with marked spatial heterogeneity in the distribution of all variables. Taller grass canopies caused competition for light between species, reducing legume contribution and inducing more erect forage peanut growth habit. Conversely, in areas with lower grass canopy height, forage peanut botanical composition increased to up to 70%, as it propagated more effectively by stoloniferous propagation.Conclusions The canopy structure spatial variability of a Marandu palisadegrass-forage peanut mixed pasture determines the overall average pasture grass/legume proportion. In regions with taller canopies, Marandu palisadegrass was favoured, while in areas with shorter canopy, forage peanut proportion was promoted. Satisfactory legume proportions can be reached in the canopy despite areas with reduced legume contribution.Implications Our findings using geostatistical techniques facilitate the development of useful and innovative tools allowing better comprehension for the management of mixed pastures. MenosContext Understanding ecology in grass-legume pastures can help support strategies aimed at maintaining canopy stability in terms of botanical composition.Aims This 2-year study evaluated spatial variability, focusing on plant structural characteristics in a Marandu palisadegrass [Urochloa brizantha (Hochst. Ex A. Rich.) R.D. Webster cv. Marandu]-forage peanut (Arachis pintoi Krapov. and W.C. Greg. cv. BRS Mandobi) mixed canopy under grazing in continuous stocking management.Methods The pasture was managed with canopy height of 20-25 cm. The experimental area had a permanent sample grid containing 50 pre-established and georeferenced plots. Principal component analysis was performed, and spatial dependence structure of the first principal component and structural variables were separately characterised by geostatistical analysis.Key results There was spatial dependence of the structural characteristics, with marked spatial heterogeneity in the distribution of all variables. Taller grass canopies caused competition for light between species, reducing legume contribution and inducing more erect forage peanut growth habit. Conversely, in areas with lower grass canopy height, forage peanut botanical composition increased to up to 70%, as it propagated more effectively by stoloniferous propagation.Conclusions The canopy structure spatial variability of a Marandu palisadegrass-forage peanut mixed pasture determines the overall average pasture grass/legume proportion. In regions wi... Mostrar Tudo |
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Palavras-Chave: |
Clonal propagation; Mixed pasture; Spatial variability; Warm season legume. |
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Thesaurus NAL: |
Arachis pintoi; Geostatistics; Urochloa brizantha. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 02938naa a2200325 a 4500
001 2153435
005 2023-04-28
008 2023 bl uuuu u00u1 u #d
022 $a1836-0947
024 7 $a10.1071/CP22134$2DOI
100 1 $aSPASIANI, P. P.
245 $aLight competition is the key factor determining spatio-temporal variability in legume proportion within Marandu palisadegrass-forage peanut mixed pastures.$h[electronic resource]
260 $c2023
520 $aContext Understanding ecology in grass-legume pastures can help support strategies aimed at maintaining canopy stability in terms of botanical composition.Aims This 2-year study evaluated spatial variability, focusing on plant structural characteristics in a Marandu palisadegrass [Urochloa brizantha (Hochst. Ex A. Rich.) R.D. Webster cv. Marandu]-forage peanut (Arachis pintoi Krapov. and W.C. Greg. cv. BRS Mandobi) mixed canopy under grazing in continuous stocking management.Methods The pasture was managed with canopy height of 20-25 cm. The experimental area had a permanent sample grid containing 50 pre-established and georeferenced plots. Principal component analysis was performed, and spatial dependence structure of the first principal component and structural variables were separately characterised by geostatistical analysis.Key results There was spatial dependence of the structural characteristics, with marked spatial heterogeneity in the distribution of all variables. Taller grass canopies caused competition for light between species, reducing legume contribution and inducing more erect forage peanut growth habit. Conversely, in areas with lower grass canopy height, forage peanut botanical composition increased to up to 70%, as it propagated more effectively by stoloniferous propagation.Conclusions The canopy structure spatial variability of a Marandu palisadegrass-forage peanut mixed pasture determines the overall average pasture grass/legume proportion. In regions with taller canopies, Marandu palisadegrass was favoured, while in areas with shorter canopy, forage peanut proportion was promoted. Satisfactory legume proportions can be reached in the canopy despite areas with reduced legume contribution.Implications Our findings using geostatistical techniques facilitate the development of useful and innovative tools allowing better comprehension for the management of mixed pastures.
650 $aArachis pintoi
650 $aGeostatistics
650 $aUrochloa brizantha
653 $aClonal propagation
653 $aMixed pasture
653 $aSpatial variability
653 $aWarm season legume
700 1 $aHOMEM, B. G. C.
700 1 $aLIMA, I. B. G. de
700 1 $aGUIMARAES, B. C.
700 1 $aMEDEIROS, E. S. de
700 1 $aMUIR, J. P.
700 1 $aOLIVEIRA, M. S. de
700 1 $aBODDEY, R. M.
700 1 $aCASAGRANDEM, D. R.
773 $tCrop & Pasture Science, Published online: 21 March 2023.
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